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fsp1 activity  (Elabscience Biotechnology)


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    Elabscience Biotechnology fsp1 activity
    Fsp1 Activity, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inhibitor+screening+kit/Ferroptosis+Suppressor+Protein-1+(FSP-1)Inhibitor+Screening+Kit/pmc13019507-425-18-20
    Average 94 stars, based on 2 article reviews
    fsp1 activity - by Bioz Stars, 2026-09
    94/100 stars

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    Activity Assay:

    Article Title: Endarachne binghamiae Extract Alleviates Colitis by Suppressing NLRP3 Inflammasome Activation via Regulation of NOX–iNOS Crosstalk
    Article Snippet: NOX activity was measured from cell lysates using a superoxide-based colorimetric assay kit (E-BC-K815-M, Elabscience, Houston, TX, USA). iNOS activity was assessed indirectly by quantifying nitric oxide (NO) accumulation in culture supernatants using the Griess reagent method. .. XO activity was determined using an inhibitor screening kit (E-BC-D019, Elabscience, Houston, TX, USAE); The percentage inhibition values provided by the assay were converted to relative activity values using the formula: relative activity (%) = 100 − % inhibition, where lower inhibition corresponds to higher residual XO activity. ..

    Article Title: Endarachne binghamiae Extract Alleviates Colitis by Suppressing NLRP3 Inflammasome Activation via Regulation of NOX-iNOS Crosstalk.
    Article Snippet: NOX activity was measured from cell lysates using a superoxide-based colorimetric assay kit (E-BC-K815-M, Elabscience, Houston, TX, USA). iNOS activity was assessed indirectly by quantifying nitric oxide (NO) accumulation in culture supernatants using the Griess reagent method. .. XO activity was determined using an inhibitor screening kit (E-BC-D019, Elabscience, Houston, TX, USAE); The percentage inhibition values provided by the assay were converted to relative activity values using the formula: relative activity (%) = 100 − % inhibition, where lower inhibition corresponds to higher residual XO activity. ..

    Inhibition:

    Article Title: Endarachne binghamiae Extract Alleviates Colitis by Suppressing NLRP3 Inflammasome Activation via Regulation of NOX–iNOS Crosstalk
    Article Snippet: NOX activity was measured from cell lysates using a superoxide-based colorimetric assay kit (E-BC-K815-M, Elabscience, Houston, TX, USA). iNOS activity was assessed indirectly by quantifying nitric oxide (NO) accumulation in culture supernatants using the Griess reagent method. .. XO activity was determined using an inhibitor screening kit (E-BC-D019, Elabscience, Houston, TX, USAE); The percentage inhibition values provided by the assay were converted to relative activity values using the formula: relative activity (%) = 100 − % inhibition, where lower inhibition corresponds to higher residual XO activity. ..

    Article Title: Endarachne binghamiae Extract Alleviates Colitis by Suppressing NLRP3 Inflammasome Activation via Regulation of NOX-iNOS Crosstalk.
    Article Snippet: NOX activity was measured from cell lysates using a superoxide-based colorimetric assay kit (E-BC-K815-M, Elabscience, Houston, TX, USA). iNOS activity was assessed indirectly by quantifying nitric oxide (NO) accumulation in culture supernatants using the Griess reagent method. .. XO activity was determined using an inhibitor screening kit (E-BC-D019, Elabscience, Houston, TX, USAE); The percentage inhibition values provided by the assay were converted to relative activity values using the formula: relative activity (%) = 100 − % inhibition, where lower inhibition corresponds to higher residual XO activity. ..



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    Image Search Results


    Copper deprivation induced by SLC31A11 knockdown triggers the upregulation of SLC7A11. ( A ) Confocal imaging of Cu-probe and Mito-tracker in the NC and SLC31A1 knockdown AsPC-1 cells. Hoechst (blue) is used as a nuclear counterstain. Mean ± SD, n = 3. Statistical significance was determined using a two-way ANOVA test. Scale bar: 2 μm. ( B ) Total Fe level in the NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( C ) Volcano plot of gene expression (the SLC31A1 knockdown [shRNA#2] versus the control; log2(fold change) ≥1; p < 0.05 between SLC31A1 knockdown and NC AsPC-1 cells. ( D ) GO analysis of differentially expressed genes between SLC31A1 knockdown (shRNA#2) and NC AsPC-1 cells. ( E-G ) Western blot analysis of the indicated protein levels in the NC and SLC31A1 knockdown AsPC-1 (E), MiaPaCa-2 (F), and CFPAC-1 (G) cells. ( H and I ) GPX4 (H) or FSP-1 activity (I) was tested in the NC and SLC31A1 knockdown AsPC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( J ) qPCR analysis of SLC31A1 and SLC7A11 gene expression in NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( K ) qPCR analysis of SLC31A1 and SLC7A11 gene expression in NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( L ) GSH level in the NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( M ) Cystine uptake level in the NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test.

    Journal: Redox Biology

    Article Title: Copper deprivation reprograms antioxidant defense to suppress ferroptosis via SLC7A11

    doi: 10.1016/j.redox.2026.104130

    Figure Lengend Snippet: Copper deprivation induced by SLC31A11 knockdown triggers the upregulation of SLC7A11. ( A ) Confocal imaging of Cu-probe and Mito-tracker in the NC and SLC31A1 knockdown AsPC-1 cells. Hoechst (blue) is used as a nuclear counterstain. Mean ± SD, n = 3. Statistical significance was determined using a two-way ANOVA test. Scale bar: 2 μm. ( B ) Total Fe level in the NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( C ) Volcano plot of gene expression (the SLC31A1 knockdown [shRNA#2] versus the control; log2(fold change) ≥1; p < 0.05 between SLC31A1 knockdown and NC AsPC-1 cells. ( D ) GO analysis of differentially expressed genes between SLC31A1 knockdown (shRNA#2) and NC AsPC-1 cells. ( E-G ) Western blot analysis of the indicated protein levels in the NC and SLC31A1 knockdown AsPC-1 (E), MiaPaCa-2 (F), and CFPAC-1 (G) cells. ( H and I ) GPX4 (H) or FSP-1 activity (I) was tested in the NC and SLC31A1 knockdown AsPC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( J ) qPCR analysis of SLC31A1 and SLC7A11 gene expression in NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( K ) qPCR analysis of SLC31A1 and SLC7A11 gene expression in NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( L ) GSH level in the NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test. ( M ) Cystine uptake level in the NC and SLC31A1 knockdown AsPC-1, MiaPaCa-2, and CFPAC-1 cells. Mean ± SD, n = 3. Statistical significance was determined using a one-way ANOVA test.

    Article Snippet: Ferroptosis Suppressor Protein-1 (FSP-1) Inhibitor Screening Kit , Elabscience , E-BC-D008.

    Techniques: Knockdown, Imaging, Gene Expression, shRNA, Control, Western Blot, Activity Assay